흰쥐 전두엽에서 올란자핀 만성 투여 후 cDNA Microarray를 이용한 유전자 발현 변화 검색

Search of Altered Gene Expression after Chronic Administration of Olanzapine in the Rat Frontal Cortex using cDNA Microarray

Objective: cDNA microarray is a convenient molecular technology that enables to search for gene expression in large scale. To explore the effect of antipsychotics on the gene expression in the brain, we applied cDNA microarray and searched for differentially expressed genes in the olanzapine-treated rat frontal cortex. Methods: We administered olanzapine (4 mg/kg/day, IP) to S-D rats for 14days, and dissected the frontal cortex to examine. We analyzed altered gene expression from microarray, and screened up- or down-regulated genes. Their changes were confirmed by RT-PCR. Results: Three down-regulated and one up-regulated genes were screened by triplicate cDNA microarray analysis. Among them, translocase of the inner mitochondrial membrane 23 (TIM23) was confirmed in RT-PCR. The expression ofTIM23 mRNA was significantly increased in olanzapine-treated rat frontal cortex. Conclusion: This is the first report of up-regulated gene expression of TIM23 by antipsychotics in the rat brain. TIM23 is the essential component of mitochondrial biogenesis. From this result, we suggest that antipsychotic effect may be related to the improvement of mitochondrial dysfunction in the brain. (Korean.J Psychopharmacol 2003: 14(4):360-366)