학술저널
Purification and Properties of Thioltransferase from Leaf Mustard
Purification and Properties of Thioltransferase from Leaf Mustard
- 강원대학교 기초과학연구소
- 기초과학연구
- 제8집
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1997.1228 - 38 (11 pages)
- 1
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Thioltransferase, which is required for glutathione (GSH)-dependent formation of deoxyribonucleotides by ribonucleotide reductase, has been identified and purified to homogeneity from leaf mustard, The isolation procedure involved four chromatography steps on DEAE-cellulose, Sephadex G-l00 (2nd), and Q-Sepharose, The final preparation was homogenous as judged by polyacrylamide gel electrophoresis, Its molecular weight was estimated to be about 15 kDa, The purified enzyme was characterized on several aspects such as optimal pH, Km, and Vmax
Abstract Introduction Materials and Methods Results and Discussion References
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