Polyamine 합성 저해제와 Polyamine이 대두 자엽 부정근의 형성에 미치는 영향

Effects of Polyamine Inhibitors and Polyamines on the Adventitious Root Formation from Soybean Cotyledons

In order to study on the effect of polyamine inhibitors and polyamines on adventitious root formation, the correlation between adventitious root formation and polyamine levels were investigated in cultured soybean (Glycine max L.) cotyledons. Adventitious root formation was inhibited in medium containing 10??-10??M polyamine inhibitors such as a-difluoromethylornithine (DFMO), a-difluoromethylarginine (DFMA), cyclohexylammonium sulfate (CHA), dicyclohexyl-amine (DCHA) and methylglyoxal-bis (guanylhydrazone) (MGBG). An inhibitory effects at 10??M MGBG were much higher than other treatments. Treatment with 10??M MGBG plus 10??M spermine led to reversal of the effects of MGBG alone. The polyamine levels were sharply increased in the first few days in each treatment compared to control. The remarkably increasing polyamine contents were observed in medium supplemented spermine