Activities of Sulfhydryl-Related and PhenylpropanoidSynthesizing Enzymes during the Germination of Arabidopsis thaliana Seeds

Activities of Sulfhydryl-Related and PhenylpropanoidSynthesizing Enzymes during the Germination of Arabidopsis thaliana Seeds

Seeds of Arabidopsis thaliana were sowed in a Murashige and Skoog's medium and incubated in a tissue culture chamber at 26.2.C. They appeared to germinate at 60-72 hours after sowing. We measured the time-course activities of sulfhydryl-related enzymes such as thioredoxin, thiol-transferase (gIutaredoxin), thioredoxin reductase, and glutathione reductase. The activities of two enzymes, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, which are involved in phenylpropanoid synthesis, were also measured and compared. Phenylalanine ammonia-lyase activity increased from 48 hours after sowing, whereas tyrosine ammonia-lyase activity increased transiently at the early stage and decreased slightly afterwards. Thioredoxin activity gradually decreased during the germination process. However, thioltransferase activity increased drastically from 60 hours after sowing. Thioredoxin reductase and glutathione reducatase increased rapidly from 36 hours after sowing. Thioredoxin activity was found to be relatively high in the dormant seeds. Keywords: Arabidopsis tha/iana, germination, glutathione reductase, phenyalanine ammonia-lyase, thioltransferase, thioredoxin