애기장대(Arabidopsis thallana) 잎 절편에서의 기관형성 특히 Naphthale neacetic acid의 농도에 따른 부정근, 모용 및 캘러스 형성과 Determination Time

Organ Formation-The Formation of Adventitious Roots, Trichomes and Calli from Leaf Segments of Arabidopsis thallana by Naphthaleneacetic acid Concentrations, and Their Determination times

The effect of auxins and cytokinins on the formation of adventitious shoots, adventitious roots, trichomes, and calli in MS basal medium was investigated in leaf segments from ecotype Columbia of Arabidopsis thallana. Adventitious shoots, adventitious roots, trichome, and calli were formed from leaf segments by a wide range of hormone concentrations and combinations. Adventitious shoots were formed respectively in treatment with 0.1 mg/L IAA and 10 mg/L BA. Adventitious roots were formed in treatments with low concentration of IAA and NAA. Trichomes and calli were formed by increasing the concentration of IAA and NAA. The optimal combination was 0.5 mg/L NAA and 0.1 mg/L BA for trichome formation, 10 mg/L NAA and 10 mg/L BA for calli formation. When NAA was treated alone in culture media, adventitious roots were formed in 0.1 mg/L, trichomes were formed in 2.0 mg/L, and calli were formed in 1.0 mg/L Inductive time for formation of adventitious roots, trichomes and calli were determined at 6,7 and 18 days respectively by periodical transfer of leaf segments from NAA containing medium to NAA free medium. Key words: Adventitious shoot, Iaa kinetin, BA 2,4-D