애기장대(Arabidopsis thaliana)의 잎과 줄기 절편체로부터 기관분화를 통한 식물체 재분화

Plant Regeneration Through Organogenesis from Leaf and Stem Explants of Arbidopsis thaliana

Shoot induction from leaf and stern explants of Arabidopsis tfr1liana was obtained when leaf explants were cultured on MS medium containing 0.15 mg/L lAA and 5 mg/L 2-jp after liquid preculture on MS medium containing 5 mg/L lAA and 0.5 mg/L 2,4- D for 7 days and also, when stem explants were cultured on MS medium containing 0.15 mg/L lAA and 5 mg/L 2-jp after liquid preculture on CP medium containing 0.5 mg/L 2,4-D and 0.1 mg/L BAP for 7 days. Callus induction from stern explants was obtained on CP medium containing 0.5 mg/L 2,4-D and 0.1 mg/L BAP in the dark at 25 t: after four weeks of culture. Shoot forming calli selected from subcultured-calli were cultured on MS medium containing 0.05 mg/L lAA and 7 mg/L 2-jp under continous illumination for 4 weeks. Shoot regenerating frequency (explants and calli regenerating at least one shoot) was more than 55%. Regenerated shoots were rooted on MS medium without phytohomones and transplanted to potting soil. Histological observations of leaf and stern explants revealed that meristematic dome was originated from massive cells of epidermal and subepidermal cells and that meristemoid from the shoot forming calli developed to shoot primordia and shoot.