활성슬러지로부터 분리한 균주의 TNT 분해능 조사

2,4,6-trinitroluene (TNT) degrading bacterium C1 was isolated from enrichment culture of the activated sludge of Chuncheon sewage treatment plant, and the effect of culture conditions on TNT removal and metabolites formation were investigated in minimal medium containing 100 mg/L of TNT, Bacterial culture of 100 ml in 250 ml Erlenmeyer flask showed a higher TNT removal rate than that of 20 ml culture in 100 ml flask. The fates of other metabolites, such as hydroxylamino-dinitrotoluene (OHAmDNT), amino-dinitrotoluene (AmDNT) and diamino-nitrotoluene were also different in both cultures. Dinitrotoluene appeared only in 250 ml flask. This phenomenon might be due to oxygen transfer and redox conditions in cultures. When the concentration of glucose was reduced to half, TNT was removed even faster, but the concentration of AmDNT maintained relatively high level. The decrease of nitrogen concentration to half delayed the TNT removal and caused the drastic changes of concentrations of OHAmDNT and AmDNT. These results indicate that the transformations of TNT and its metabolites were more sensitive to the change of nitrogen concentration than that of carbon source, and TNT did not serve as an important nitrogen source.