Enterobacter cancerogenus에 의한 2,4,6-trinitrotoluene(TNT)의 생분해에 미치는 환경요인의 영향

Effects of various carbon and nitrogen sources on TNT degradation by Enterobacter cancerogenus isolated from activated sludge was investigated. Hydroxylaminodinitrotoluenes (OHAmDNTs), aminodinitrotoluenes (AmDNTs) and diaminitrotoluenes (DiAmNTs) were detected as main TNT metabolites in three different carbon sources (glucose, fructose, and sucrose), Although the transfonnation rates of TNT were similar with all three carbon sources, the transformation rates of OHAmDNTs were higher with glucose and the transformation rates of other metabolites such as AmDNTs and DiAmNTs were higher with. fructose. The addition of sodium nitrate showed the higher transformation rates of TNT and its metabolites compared to those with ammonium sulfate. The aerobic and anaerobic TNT degradation by E cancerogenus were also compared. In addition to the main metabolites described above, various unknown metabolites were produced in anaerobic degradation. The transformation of TNT to OHAmDNTs was fast in anaerobic culture than that in aerobic culture, but the transformation of OHAmDNTs to next metabolites was very slow.