동전점 전기영동에 의한 서해산 해산어의 근형단백질의 전기영동에 관한 연구

Electrophoretic Patterns of Sarcoplasmic Proteins in Mid-West Korean Sea Fishes by Thin Layer Polyacrylamide Gel Isoelectrofocusing

In order to Identify hard diatinct 12 fish species(shiba shrimp Metapenaeus joyneri, fleshy shrimp Penaeus orientalis, ridgetail prawn Palaemon carinicauda, yellow eroaker Pseudo-sciaena manchurica, croaker Nibea albiflora, Collichthyes fragilis, brown sole Limanda herzensteini, frog flounder Pleuronichthys cornutus, Areliacus rhomaleus,stone flounder Kareius bicoloratus, harvest fish Pampus argenteus, flag fish Goniistius zonatus) by seeing with naked eye in Kunsan coastal area, sarcoplasmic protein in the supernatant was used for isoelectric focusing. For getting supernatant, fish muscle tissue was blended with two times deionized water and centrifuged ( at 4℃, 12,000rpm for 15min). Isoelectric focusing of sarcoplasmic protein carried out on a LKB Multiphor Ⅱ using polyacrylamide gel plate(2mm thickness, pH 3.5~10℃, pH 5~8 gradient, at 10℃ for 1.5, 3 hours). In case of uncertain protein, pH gradient was modified to narrow pH gradiet, and excuted 2-D electrophoresis using conventional polyacrylamide gel electrophoresis. Most of fishes except yellow croaker and Collichthyes fragills were distingushed by isoelectric focusing. The protein maps of 2-D electrophoresis for analysing two protein bands at similar positions(pH 5, 6) between the two fish species showed the differences of the estimated molecular weights, 11,700(pH 5.0) and 87,000(pH 6.0).