넙치(Paralichthys olivaceus)육의 사후 조기 변화 3. 치사 방법이 ATP 관련 물질과 유산 함량의 변화에 미치는 영향

Early Changes after Death of Plaice, Paralichthys olivaceus Muscle

To clarify the effects of killing methods on biochemical changes of plaice, Paralichthys olivaceus muscle at early period after death. cultured plaices were killed by the following four different methods: 1. spiking at the brain instantly. 2. letting them to die in the air 3. dipping in sea water including anesthetic. 4. electrifying in sea water. Immediately after death. the changes in ATP and its related compounds. ATP breakdown. and IMP or lactate accumulation rates of muscle during storage at 5℃ were studied. ATP in samples killed by letting and electrifying were decomposed more rapidly than spiking and dipping samples. The rate constant of ATP breakdown were O.429h 1 for samples killed by electrifying. 0.224h-¹ for letting samples, 0-195h-¹ for spiking samples, and O.167h-¹ for dipping samples. The maximum speed and content of IMP or lactate accumulation were showed in samples killed by electrifying among the all killing methods. The rate constant of lactate accumulation were 2.256h I for samples killed by electrifying. 1.123h ¹ for letting samples. O.534h ¹ for spiking samples, and 0.526h-¹ for dipping samples. From the results above, it was revealed that electrifying in sea water could accelerate ATP breakdown and accumulation of IMP or lactate among the all killing methods. The other hand, dipping in sea water including anesthetic delayed those changes.