한국산 동자개(Pseudobagrus fulvidraco)의 백혈구의 분류와 미세구조

Classification and Ultrastructural observation of Leucocytes in Korean bullhead fish (Pseudobagrus fulvidraco)

Classification and ultrastructural observation of peripheral leucocytes in Korean bullhead fish(Pseudobagrus fulvidroco) were performed. The leucocytes were classified into four types of cells on the basis of their morphological, cytochemical and ultrastructural characteristics. Neutrophils were round and 7.5-12.5㎛ in diameter with round, ova! or 2 segmented nucleus. Monocytes were round or irregular cells and 7.5-12.5㎛ in diameter with bean-shaped or irreular nucleus. Lymphocytes were round and 4.5-8.0㎛ in diameter with large round nucleus. Thrombocytes were ova! or spindle shaped cells with ova! or spindle nucleus. The ceUs with greyish cytoplasm ranged 4 to 9 .urn in length and 2 to 4 ㎛ in width. Neutrophils showed positive reactions in peroxidase, PAS and ACP. Monocytes showed weekly positive reactions in ACP and PAS. Lymphocytes showed positive reactions in PAS and ACP. Thrombocytes showed positive reactions in PAS. In electron microscopic observation, nucleus of neutrophils could vary from round to oval or horse-shoes-shaped. The cytoplasm contained azurophilic garnuies, specific garnules, rough endoplasmic reticulum (RER), mitocondria, vacuoles and glycogen. Most monocytes were of the same size as neutrophils, but their shape were often more irregular. The cytoplasm contained azurophilic garnules, HER, mitocondria, vacuoles and glycogen. The lymphocytes with a large rounded or indented nucleus had scarce cytoplasm with a few mitocondria and HER. Most thrombocytes were of the same shape as nucleus. The cytoplasm contained a few mitocondria, azurophilic garnules and many vacuoles. As the results it is concluded that peripheral I leucocytes of Korean bullhead fish could be classified as lymphocytes, thrombocytes, neutrophils and monocytes.