홍삼추출물이 마우스 면역반응에 미치는 영향

Effect of red ginseng extracts on the immune response in mouse

In the present study, the effects of treatment with Red ginseng extracts (RGEs) on immune response such as cellular and humoral immune reaction were measured in balb/c mice in vivo. An aqueous extract of ginseng was treated by oral administration at a dosage of 10㎍/g of body weight/day for 5 days before or after SRBC sensitization. We further attempted to measure the effects of the treatment of RGEs directly for function of mononuclear cells such as lymphocyte proliferation and macrophage function such as phagocytosis, NBT reduction and bacteriocidal effects. The treatment of RGEs enhanced the arthus reactions, delayed type hypersensitivity, antibody productions, rosette forming rates of splenocytes and spleen indices compared to untreated control group, especially in the group treated after SRBC sensitization significantly. DTH reaction was most significantly activated in the all treatment groups; indicating that RGEs enhanced the immune response via cellular mediated immunity. The proliferation lymphocytes of co-cultured with T-cell mitogen, Concanavalin A (Con A) or phytohaemagglutin (PHA-P), was significantly inhibited in a dose-dependent manner. Contrary to this inhibited effect, no significant differences were found In B cell mitogen lipopolysaccharide (LPS) treatment. With comparison, the treatment of ginseng extract alone slightly increased the' proliferation with no significance. Bacteriocidal activity of peritoneal macrophages was somewhat increased in all treated groups than in control, especially at the concentration of lOJLg/ml and this augmentation was due to the increase of phagocytic activity and super oxide anion production. These results led conclusion activation of that the to macrophage function might be chiefly or partially due to the enhancement of the immune system.