가시파래 수용성 다당의 추출 및 화학적 조성

Extraction and Chemical Composition of Soluble Polysaccharide from Green Laver, Enteromorpha prolifera

Soluble polysaccharide (SP) from green laver, Enteromorpha prolifera was extracted 3 times with distilled water at 100℃ for 2 hrs and fractionated with cetylpyridinium chloride (CPC) and ion exchange chromatography (DEAE Separose CL-6B). The SP amounted to 23.7% of the dry seaweed weight and contained 68.8% carbohydrate, It was mainly constituted of rhamnose, glucose, xylose, sulfate and uronic acid and was fractionated with CPC into three (CPC-S, CPC-PS, CPC-PP) fractions. The major acid fraction CPC-PS accounted for 10.2% of the dry algal weight. CPC-PS was further fractionated on DEAE Sepharose CL-6B into Fr-l (8.0%), Fr-2 (35.8%), Fr-3 (23.7%) fractions, The Fr-3 fraction contained 2.2% protein, 21.4% sulfate, 15.3% uronic acid, and 72.4% polysaccharides composed of rhamnose, xylose and glucose. The Fr-2 fraction, which was richer in uronic acid (17.5%) and poorer in sulfate (19.0%) .and total sugar (68.8%) than the Fr-3, had a sugar composition close to that of Fr-3. The average molecular weights of Fr-2 and Fr-3 were 510,000 and 830,000 daltons, respectively. Fr-3 turned out to be homogeneous by cellulose acetate electrophoresis.