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학술저널

가시파래 산성 수용성 다당의 구성당 결합 특성

Glycosyl-linkages of Acid Soluble Polysaccharide from Green Laver, Enteromorpha prolifera

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Green laver, Enteromorpha prolifera, is regarded as one of important materials for food processing in Korea. The acidic water-soluble polysaccharide (CPC- PS) isolated from the alga with hot water and cetylpyridium chloride was mainly constituted of rhamnose, xylose, uronic acid and sulfate. To determine the glycosyl-linkages and positions of sulfate by methylation, the CPC- PS was reduced and/or sulfated. A marked increase of glucose content in the reduced polysaccharide indicated that glucuronic acid was a major sugar in the polymer and sulfation was deduced to occur on 03 of rhamnose and 02 of xylose. According to the methylation analysis of the native, reduced, desulfated and reduced-desulfated polymers, CPC- PS mainly composed of 1,4- and 1,2,3-linked rhamnose 3-sulfate, 1,4-linked xylose 2-sulfate, l,4-linked xylose and 1,4-linked glucuronic acid. Minor 1,4-linked rhamnose and 1,4,6-linked galactose residues were also detected.

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