RBL―2H3 세포에서 Fluoxetine에 의한 Tryptophan Hydroxylase 활성도 변화

Fluoxetine-induced Changes on Activity of Tryptophan Hydroxylase at RBL-2H3 Cells

Objectives The main of the present study is to explore the effect of fluoxetine on transcription, translation and activity of tryptophan hydroxylase (TPH), and intracellular level of serotonin. Methods. The expression level of the TPH mRNA and the protein, the TPH enzyme activity, and the intracellular level of serotonin were explored at the fluoxetine-treated RBL-2H3 cells. Real-time RT-PCR and immunoblotting analysis confirmed changes in the expression of TPH mRNA and protein The activity of TPH was measured using [³H]tryptophan The intracellular level of serotonin was measured by HPLC Results The TPH activity was gradually increased on time from 24hr to 72hr. The real-time RT-PCR also revealed that the TPH mRNA was increased at 12, 24 and 72hr in the fluoxetine-treated RBL-2H3 cells. The Immunoblotting analysis also revealed that the TPH protein was decreased at 72hr in the fluoxetine-treated RBL-2H3 cells The intracellular level of serotonin was increased at 48hr after treatment of fluoxetine. Conclusion Fluoxetine induced the increases of the TPH mRNA, the TPH enzyme activity and intracellular level of serotonin, and the decrease of the TPH protein expression at the RBL-2H3 cells. (Korean J Psychopharmacol 2004;1S(4):449-456)