흰쥐 C6 신경교종 세포에서 Fluoxetine을 장기간 처리 시 발현이 조절되는 유전자의 규명

Characterization of Differentially Expressed Genes upon Chronic Fluoxetine Treatment in Rat C6 Glioma Cells

Objective: The aim of this study was to identify diffrentially regulated genes after the treatment of fluoxetine in rat C6 glioma cells using cDNA microarray chip techniques and real-time RT-PCR Methods, Cells were Incubated for 24 hours, and for 72 hours with or without 100M fluoxetine. Total RNAs extracted from cells were reversely transcribed to cDNA These cDNA were used to carry out cDNA microarray chip. A part of the up-/down-regulated genes in cDNA microarray result were confirmed by real-tune RT -PCR Results 1) Genes in fluoxetin cells for 72 hours (chronic treatment) were more regulated than that in fluoxetine-treated cells for 24 hours (acute treatment). 2) The expression level of Gs gene in fluoxetine-treated cells for 24 hours hardly altered, but that of Gs In fluoxetine-treated cells for 72 hours significantly increased. The expression of G12 also decreased ill 72 hours In relation to 24 hours after the administration of fluoxetine. 3) The expression level of NCAM140 gene in fluoxetine-treated cells was higher than that in control cells Conclusion We Identified genes (Gs, Gi2 and NCAM140)related to neural plasticity and Intracellular signal transduction cascade from our result this Implies that fluoxetine may inhibit atrophy or death of impaired neural cells by promoting neurite outgrowth. (Korean J Psychopharmacol 2004;15(4):457-467)