Laser scanning confocal microscope (LSCM)-fluorescence spectral properties of Nile Red embedded in polystyrene film of different thickness

Laser scanning confocal microscope (LSCM)-fluorescence spectral properties of Nile Red embedded in polystyrene film of different thickness

  The ensemble and single molecule (SM) fluorescence properties of Nile Red embedded in polystyrene (PS) films on glasses were investigated. The ensemble spectra showed dual emission bands and SM spectra exhibited distribution with two emission maxima. These results indicate that two distinct environments exist in the thin PS film on a glass: a low polarity environment in the PS bulk and a high polarity environment near the PS/g1ass interface. It was also found that a PS film of ca 10 ㎚ thick is sufficient to separate fluorophores from the charged species on glass surfaces.