RAPD산물의 Direct Oligonucleotide Extension Sequencing을 이용한 쇠고기 품종 구분용 SCAR marker의 개발

Development of a SCAR Marker by Direct Oligonucleotide Extension Sequencing(DOES) of RAPD products : a practical tool for Identification of Beef Breeds

  Random amplified polymorphic DNA (RAPD) is widely used technique for genetic fingerprinting, but the practical applications of such markers are enhanced by sequencing the RAPD products and converting them more specific SCAR (sequence-characterized amplified region) markers. We describe on efficient method for directly sequencing purified gels  which containing RAPD fragments amplified by using one primer from a set of four 3"-terminal extended oligo nucleotides, which is called direct oligonucleotide extension sequencing(DOES). Several RAPD bands were converted into dominant SCAR markers by direct sequencing, which is avoiding the costly and time consuming cloning step. We have used this technology to develop SCAR markers useful for identifying the beef breeds(Korean cattle beef, Imported beef).