8명의 한국인 남자 학생들에서 Sodium Valproate의 생체이용률 연구를 위한 HPLC 분석 방법의 개발

A Simple HPLC Analysis for the Study on Bioavailability of Sodium Valproate tablets in 8 Korean Male Students

  Sodium Valproate levels are frequently monitored in clinical laboratories to assess compliance in epileptic patients and to optimize theraphy. In this study, a simple and accurate high performance liquid chromato-graphic method for valproic acid in plasma was developed to be applicable for the studies on the bioavailability of sodium valproate tablets and the therpeutic drug monitoring of plasma valproic acid level. All plasma samples were added with an internal standard, zinc sulfate solution, and acetonitrile, followed by centrifugation. The supernatants were added with 4-bromophenacyl bromide and were heated at 70℃ for 15min. The derivatization products were extracted with hexane, and the hexane extraction solutions were evaporated to dryness and redissolved in mobile phase. The solution was analyzed using HPLC system. The inter-Day and the intra-Day coefficients of variation ranged from 1.8% to 4.9%, and 3.0% to 4.2%. The simple and accurate HPLC method of analysis of plasma valproic acid seems to be adequate for the studies of bioavailability of sodium valproate tablets and therapeutic drug monitoring of plasma valproic acid level.