활성산소와 휘발성유기화합물류에 대한 노출이 세포 증식에 미치는 영향에 대한 연구

Effects of Reactive Oxygen Species and Volatile Organic Compounds on the Cell Proliferation

  Oxidative stress is easily induced by volatile organic compounds. The adverse health effects of a number of environment pollutions are related to the formation of free radicals and oxidative stress. Induction of antioxidant defensive system in the response to an oxidative attack is an essential element of the organism to survive. Oxidative stress includes formation of hydrogen peroxide. Volatile organic compounds are constituents of numerous industrial and commercial products such as solvents, paints and unleaded gasoline. Their high toxic potential and liposolubility make these compounds important health hazards. The aim of the present study was to investigate the effect of reactive oxygen species and volatile organic compounds on the cell proliferation. Cell proliferation was measured after 24h treatment with volatile organic compounds such as benzene, xylene, chloroform, propanol, hexane or tetrachloroethylene. The cell proliferation was not modified after exposure of benzene(100 mM), propanol(100 mM) or hexane (100 mM). Treatment of xylene(100 mM), chloroform(100 mM) and tetrachloro-ethylene(100 mM) strongly inhibited the cell proliferation. However, treatment of 10 mM xylene, 10mM chloroform and 10mM tetrachloroethylene showed no effects on cell proliferation. Cell proliferation was also measured after 24 h co-exposure of volatile organic compounds and hydrogen peroxide. Addition of hydrogen peroxide did not modify the cell proliferation pattern induced by volatile organic compounds. Any additive effect of hydrogen peroxide was not observed. These results show that volatile organic compounds induce inhibition of cell proliferation with different potency. Future studies will concentrate on the mechanism of the chemical interaction.