학술저널
High-Resolution Melting Assay for the Detection of fliC variations in Salmonella serotypes Dublin and Naestved
High-Resolution Melting Assay for the Detection of fliC variations in Salmonella serotypes Dublin and Naestved
- 고려대학교 보건과학연구소
- 보건과학논집
- 保健科學論集 Vol.32 No.2
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2006.1267 - 72 (6 pages)
- 19
High resolution melt curve analysis(HRM) is a simple post-PCR technique which can be used for mutation scanning and genotyping. We identified Salmonella enterica Dublin and S. enterica Naestved which have single base difference between them in fliC gene by HRM. The 102-base pair amplicons expressed different melting temperatures. Using the method, we can identify serotypes even which have a base variant without post-PCR handling, resulting in short analysis time.
Abstract<BR>Ⅰ. Introduction<BR>Ⅱ. Materials and Methods<BR>Ⅲ. Results and Discussion<BR>References<BR>
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