참외의 엽산 분석방법 및 함량

Folate Assay Procedure and Content of Muskmelon

  Trienzyme treatment (protease, α-amylase, folate conjugase) before microbiological assay is widely used to measure folate content in foods. The amounts of protein and carbohydrate in foods, however, are varied and the condition of enzyme treatment should be adjusted by food composition. The purpose of this study was to determine the optimal condition of trienzyme treatment for folate assay in muskmelon. The lowest concentration of protease to digest protein in muskmelon was tried to be determined because muskmelon contains only a small amount of protein. Protease and heat treatment did not increase folate assay results of muskmelon at all, which indicates these steeps are not necessary. Therefore, folate content of muskmelon was measured after the treatment of α-amylase and folate conjugase without protease treatment. The average folate content of muskmelon including placenta was 87.5㎍/100g. Folate content of placenta of muskmelon (260.7㎍/100g) was about 6 times higher than flesh alone (64.2㎍/100g). Our results show that muskmelon, especially with placenta, is a good source of folate.