제주도 토착방선균의 UV-B 손상 사람 피부각질세포(HaCaT cell) 재생효과에 관한 연구

Regeneration effect by Jeju Island endemic Actinomytes against ultraviolet-B induced damage of the HaCaT cell

&nbsp;&nbsp;It has been known that Actinomycetes have a strong biological activities against various types of the human disease. To investigate whether Actinomycetes from Jeju Island have protective effects on ultraviolet B induced apoptosis of the human skin cells, 459 species of the Streptomyces spp. were separated from the soil of Jeju Island and bred in AGS and Bennet agar medium. HaCaT cells were cultured and exposed to the UV-B illumination for 3J/㎡. After 24 hours, the Streptomyces culture extracts were added to the culture medium for another 48 hours to investigate the protective effect.<BR>&nbsp;&nbsp;The result were as follows: More than 40% of cell regeneration effect was shown in 32 species extracts including CB6, 7, 11, 24, 28, 29, 34, 39, 40, 76, 81, 121, 124, 135, 150, 188, 192, 198, 200, 215, 216, 256, 274, 275, 277, 288, 293, 294, 323, 439, 440, 473 out of a total of 459 species Streptomyces spp. extracts. This result indicated that the growth of the HaCaT cell damaged by ultraviolet ray B was prompted. Among them, 9 species showed over 80% of cell regeneration effect, and especially, CB150 showed the highest cell regeneration effect out of those surveyed with 89.7 ± 0.9%.<BR>&nbsp;&nbsp;In conclusion, Actinomycetes from Jeju Island showed strong protective effects on UV-B irradiation in human skin cells.