운동기술훈련이 태아알코올증후군 흰쥐 모델의 소뇌 발달과 운동기능에 미치는 영향

The Effect of Motor Skill Training on Motor Function and Cerebellar Development After Alcohol Exposure in Neonatal Rats

The purpose of this study was to test that motor skill training enhance motor function and cerebellar development. Using an animal model of fetal alcohol syndrome-which equates peak blood alcohol concentrations across developmental period-critifical periods for the effect of alcohol on body and cerebellar weigh was examined. The effect of motor skill training on motor function and cerebellar developmet of rat exposed alcohol on postnatal days 4 through 10 were studied. Newborn rats were assigned to one of two groups: (1) Control group (CG), via artificial rearing to milk formula and (2) experimental groups (EG), via 4.5g/㎏/day of ethanol in a milk solution. After completion of the treatments, the pups were fostered back to lactating dams, and wearing they were raised in standard caged until they were postnatal 48 days. Rats from experimental group of postnatal treatment then spent 10 days in one of two groups: Experimental group Ⅱ (EGⅡ) was had got motor skill training (training traverse a set of 6 elevated obstacles) for 4 weeks. Experimental group Ⅰ (EGⅠ) was not trained. Before sacrificing, the rat got examined two behavioral test, body weigh and cerebellar weigh, then coronal sections were processed. The section was investigated the Purkije cell in the cerebellum using light microscope. The results of this study were as follows. 1. In body weight test, the outcome of alcohol groups were significantly lower than the normal group. 2. In cerebellar weight test, the outcome of EGⅠ were significantly lower than CG and EGⅡ. 3. In motor behavioral test, the outcome of EGⅠ was significantly lower than NG and EG Ⅱ. 4. In Purkinje cells counting test, the outcome of EGⅠ was significantly lower than the NG and EG Ⅱ. These result suggest that improved motor function induced by motor skill training after postnatal exposure is associated with dynamically altered expression of Purkinje cells and that is related with cerebellar function. Also, these data can potentially serve as a model for therapeutic intervention.