For cutting in vitro, culture conditions of tea plants, monthly contamination rate, selection of disinfectants and infectant exposure time, were investigated. Monthly contamination rates were 12% in May, 15% in Feburuary, 32% in November and 37% in September in vitro conditions. Therefore, it was considered that optimum cutting time was from March to April. The disinfectant of 95% ethanol and 7--8% CaOCl treated was heavily contaminated by 8~9% than 95% ethanol and 25% NaOCl treated by 3% but in the latter case, survival rate was reduced and the disinfective degree was reduced in low concentration. Contamination rates on disinfectant exposure time were 10~16% in the 95% ethanol and CaOCl treated for 15~20min and decreased by 9~11% in the 95% ethanol and 25% NaOCl for 15~20min. but necrosis rate of the latter was 18~79% and survival rate was reduced. The optimum disinfection conditions in vitro culture tea plants are as follows ; after dip plant in the 95% ethanol for 3~5 seconds, disinfect by 7~8% CaOCl treated for 15~20min. and rinse in sterilized water, 3~5 time repeatly.
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