학술저널
Simple reproducible procedures are described for assessing freezing injury of tea leaf involving controlled freezing of excised leaves and measurements of leached electrolytes and reduction of 2, 3, 5-triphenyl tetrazolium chloride (TTC) to a water~insoluble red formazan. The results showed that two methods had highly significant linear regression (R² = 0.757), suggesting TTC test had an advantage that a small amount of tissue (30~80 mg) is required. The two methods can be used in selection and breeding for freezing resistance in tea.
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