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國內 野生茶의 잎과 줄기 절편 培養에 따른 Callus 誘導

Callus Induction of Korean Wild Tea Plants by leaf and stem culture

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For callus induction of Korean wild tea plants, leaf and stem segments were cultured on MS medium supplemented with different phytohormones. 1) The stem segments were heavily contaminated than the leaf segments, however, survival rates of the callus induced from stem segments were higher than those from leaf segments. 2) The callus induction rates from leaf segment were 42.2%, 35.2%, and 30.0% for 1st, 2nd, and 3rd leaf, respectively. 3) The callus induction rates from stem segment were higher in younger stems than in older ones. The induction rates were 58.4%, 56.0%, 50.0%, 45.2%, and 30.0% for 1st, 2nd, 3rd, 4th, stem and epidermal layer, respectively. 4) The callus induction rates from the epidermal layer were less than 60% of those from the intact stems. 5) The callus was more effectively induced when MS medium was supplemented with a combination of IBA and BA than with 2, 4-D and NAA.

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