To investigate the optimal conditions for shoot multiplication from node culture in tea plant (Camellia sinensis (L.) O. Kuntze), the effects of plant growth regulators, medium and sucrose were tested. Shoot proliferation was largely affected by plant growth regulators. Among plant growth regulator treatments, the best shoot multiplication was obtained at 3.0.mg/L BA and 0.5mg/L IBA combination treatmet, which produced normally elongated 4.6 shoots. In proportional to BA concentration increment, shoot length was reduced. Comparing medium kinds, MS and 1/2MS medium was superior to WPM on shoot number and growth. On sucrose concentration treatments, 30g/L sucrose was effective on shoot number and fresh weight. Consequently, these results showed that shoot multiplication through tea node cultures could be successfully carried out on MS medium supplemented with 3.0mg/L BA. 0.5mg/L IBA, and 30g/L sucrose.
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