The efficient micropropagation system using shoot cultures of tea plant(Camellia sinensis (L.) O. Kuntze) was established. For shoot multiplication and root formation, effects of plant growth regulators, medium and sucrose were investigated. Among BA and IBA combination treatments, the highest number of shoot was obtained with 2.0mg/L BA and 0.2mb/L IBA. MS medium was more effective on shoot length, shoot number, fresh weight than 1/2MS and WPM. Shoot multiplication was more effective on 30g/L sucrose than 20g/L and 40g/L. The optimal concentration for the rooting of shoot cuttings was 3.0mg/L IBA, in which 88.9% rooting frequency was obtained. The plantlets were transferred to soil mixtures with vermiculite and perlite, and successfully acclimatized.
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