혈장 및 세포의 기질에 존재하는 당단백인 Fibronectin은 여러 각막질환치료에 유효함이 밝혀져 있다. 본 저자들은 20대~60대의 성인 35명(남: 19명, 여: 16명을 대상으로 Gelatin-coupled Sepharose 4B 친화성 크로마토그래피 및 Sephadx G-25M 젤여과를 통해 Fibronectin을 분리정제하는데 성공했으며 정제된 Fibronectin이 면역화학적 실험결과 다른 혈장단백의 오염이 없는 순수한 것임을 증명할 수 있었다. 혈장내 Fibronectin의 농도는 288.9±80.2㎍/ml였고 점안제의 농도는 294.3±47.4㎍/ml였다. 통계학적 처리상 남녀간의 차이는 없었으나 연령 증가에 따른 증가를 보여주었다.(p<0.05). 정제에 소요되는 시간은 약 40분 정도였으며 생물학적 활성의 유지는 4°C냉장보관시 약 4주까지 보여주었다.
Fibronectin(FN), a glycoprotein present in plasma and extracellular matrix, has been reported to be effective on carious cornel disorders such as persistent epithelial defect, corneal trophic ulcer, herpetic keratitis, etc. We performed the study of the preparation of purified FN from 35 persons plasma(male:19, female: 16)by the use of Sepharose 4B Affinity Chromatography and Gel filtration. The Prepared FN was pure electrophoretically, and no other plasma proteins were contaminated, and confirmed as pure by multiple methods such as SDS-PAGE, Ouchterlony double gel diffusion and immunoelectrophoresis. Only 40 minutes was taken for preparation. The result showed that FN concentrations in plasma and in prepared solution were 288.9±72.8㎍/ml and 294.3±47㎍/ml respectively. FN concentrations in plasma and FN eyedrops showed no difference between sex, but increased their level with age(p<0.05). The biological activity was better preserved at 4°C(in the refrigerator) than at room temperature till about 4 weeks after preparation. Applicability of this method as a useful preparation of purified FN is recommendable.
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