마우스 종양세포의 세포독성에 미치는 인삼 추출액과 방사선조사의 병용 효과

Cytotoxic Effect of X-irradiation of Mouse Tumor Cells in the Presence of Korean Ginseng Extract

목 적 : 수용성 인삼 추출물의 현저한 세포독성에 관한 저자의 이전 실험결과를 토대로 하여 본 연구에서는 인삼과 방사선의 병용처리가 종양세포의 세포독성에 미치는 영향을 살펴보고자 하였다. 대상 및 방법 : 고려인삼 50 g과 증류수 1 L를 혼합하여 100℃에서 5시간 동안 열탕 환류추출한 다음 여과하였다. 이 여액을 원심분리한 후 동결건조하여 수용성 인삼 추출물 시료로 하였다. 방사선조사는 6 MeV 선형가속기를 이용하였고, 인삼의 세포내 독성은 마우스 섬유육종세포의 생존을 감소시키는 능력으로 평가하였으며, 인삼 1 mg/mL를 방사선조사 1시간전 종양세포에 접촉시켰다. 결 과 : 환류추출과 동결건조에 의해 최종적으로 얻어진 고려인삼 50 g의 수용성 추출물은 3.13 g으로서 수율은 6.3% 이었다. 인삼 추출액의 시험관내 세포독성 지표로서 0.001, 0.01, 0.1 및 1 mg/mL에서의 생존분율은 각각 0.89±0.04, 0.86±0.06, 0.73±0.1, 0.09±0.02로 나타났다. 방사선조사 단독의 경우 2, 4, 6 및 8 Gy에서의 생존분율은 0.81±0.07, 0.42±0.08, 0.15±0.02, 0.03±0.01으로 나타났으며, 인삼 추출물(0.2 mg/mL)과 방사선조사의 병용시 동일 조사량에서의 생존분율은 각각 0.28±0.01, 0.18±0.03, 0.08±0.02, 0.006±0.002 이었다( p<0.05). 결 론 : 환류추출과 동결건조 과정을 통해 얻어진 고려인삼 수용성 추출물의 수율은 6.3%이었다. 방사선조사시 인삼을 병용한 경우, 종양세포의 세포독성이 방사선조사 단독의 경우 보다 부가적으로 증가하였다.

Purpose : We already reported the results that aqueous extract of Korean ginseng roots showed a marked cytotoxicity. In this study, we investigated whether combined ginseng product with X-irradiation increase the cytotoxicity of tumor cells than X-irradiation or not. Materials and Methods : Fifty gram of Korean ginseng powder mixed with 1 L of distilled water was extracted with reflux flask under condition of 100℃ for 5 hrs. This aquaous ginseng extract was filtered, centrifuged and then was freezed under condition of -90℃ for 16-18 hrs. The freezing extract was dried with freeze drier, and then diluted. X-irradiation was given to tumor cells by 6 MeV linear accelerator. The cytotoxicity of ginseng in vitro was evaluated from its ability to reduce the clonogenecity of fibrosarcoma (FSa II) cells. In X-irradiation alone group, each 2, 4, 6 and 8 Gy was given to tumor cells. In X-irradiation with ginseng group, 0.2 mg/mL of ginseng extract was exposed to tumor cells for 1 hour before X-irradiation. Result : The yield for 50 g of ginseng extract which was treated with freezing drier was 3.13 g(6.3%). Cytotoxicity in vitro was measured as survival fraction which was judged from the curve, at ginseng concentration of 0.001, 0.01, 0.1 and 1 mg/mL were 0.89±0.04, 0.86±0.06, 0.73±0.01 and 0.09±0.02, respectively. Survival fraction at X-irradiation alone of 2, 4, 6 and 8 Gy were 0.81±0.07, 0.42±0.08, 0.15±0.02, 0.03±0.01, respectively. But, survival fraction in combined group of X-irradiation and ginseng (0.2mg/mL) at each same radiation dose were 0.28±0.01, 0.18±0.03, 0.08±0.02, 0.006±0.002, respectively ( p<0.05). Conclusion : The yield for ginseng extract which was treated with freezing drier was 6.3%. Cytotoxicity of Fsa II in combined ginseng with X-irradiation group was increased than that of X-irradition alone group, and its enhancing effect seemed to be added.