비만세포의 안정화작용을 가진 상백피가 colchicine에 의하여 유도되어지는 비만세포의 활성화를 억제하는지 알아보고 그 기전이 무엇인지를 파악하기 위하여 비만세포의 형태변화, 히스타민 유리 및 칼슘유입에 대한 실험을 실시하여 다음과 같은 결과를 얻었다. 1. Colchicine은 비만세포의 형태를 원형이나 난원형에서 세포질의 한쪽 또는 양쪽 극이 볼록해지는 표주박모양, 땅콩모양 등으로 변화시켰으며, 이런 비만세포의 핵은 세포의 중심에서 외곽으로 이동되어 있었다. 그러나 상백피를 전처리한 결과 이러한 현상이 70% 이상 억제되었다. 2. Colchicine은 흰쥐 복강 비만세포로부터 히스타민유리와 칼슘유입을 유도하였으나, 정상 대조군과 유의성있는 차이는 없었으며 상백피를 전처리한 결과 히스타민유리와 칼슘유입이 억제되었다. 이상의 결과로 보아 상백피는 비만세포내로의 칼슘유입을 억제함으로써 colchicine에 의한 비만세포의 형태변화와 히스타민 유리를 억제하는 것으로 생각된다.
Cortex mori (Morus alba L. : Sangbaikpi), the root barks of mulberry tree, has been used as an antiphlogistic, diuretic, and expectorant in herbs. Previous studies have demonstrated that the phenolic extracts of Cortex mori have hypotensive, hypoglycemic, antifungal, antiviral, antiinflammatory, and anticancer effects, and the hot water extract from Cortex mori has the inhibitory effects on compound 48/80-induced mast cell degranulation and histamine release from rat mast cells (RMCs). Colchicine, an alkaloid found in the crocuslike plant, has been known as an inhibitor of mitosis and microtubule assembly by formation of tubulin-colchicine complex. The purpose of this study was to examine the effect of Cortex mori on the colchicine-induced mast cell degranulation, histamine release, and calcium uptake as a part of the study of the role of microtubules in the mast cell secretory process and the inhibitory mechanism of Cortex mori. The results were summarized as follows. Cortex mori sigif icantly inhibited the colchicine-induced cytomorphological changes of RMCs such as displacement of the nucleus to the periphery, pronounced anisodiametry of the cytoplasm, stubby or broad cytoplasmic mass. Colchicine induced histamine release from RMCs and calcium uptake into RMCs, but it was not signif icantly , compared to those of negative control group. Cortex mori inhibited the colchicine-induced histamine release from RMCs and calcium uptake into RMCs. Above results, it is suggested that Cortex mori has an activity to inhibit the colchicine-induced cytomorphological changes by inhibition of calcium uptake into the mast cells.
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