한국재래산양의 임신 30, 45, 60, 90, 95, 105, 120일령 태아 및 신생아의 끝뇌를 대상으로 면역조직화학적 방법을 이용하여 GFAP 면역반응세포의 분포를 연구하여 아래와 같은 결과를 얻었다. 1. GFAP 면역반응세포가 처음 관찰되는 시기와 부위는 임신 45일령태아의 대뇌겉질, 대뇌속질, 가쪽뇌실부위였으며, 이 때 돌기형태는 무극형이었다. 2. 부챗살아교세포의 돌기변화는 시간변화에 따라 무극형이 두극형으로 그리고 홑극형으로 변한 다음 뭇극형으로 변형 되었으며 두극형면역반응세포들은 돌기들을 뇌실에서 연질막으로 뻗은 다음 연질막부위에서 종말발로 확장되어 있었다. 3. 태아에서 뭇극형 면역반응세포가 관찰되는 시기는 대뇌겉질에서 임신 60일령, 대뇌속질에서 임신 95일령에서였으며 가쪽뇌실에서는 임신 105일령의 뇌실막아래구역에서였다. 4. GFAP 면역반응세포의 수는 임신 45일령태아에서 임신 90일령태아까지 증가하다 105일령태아까지는 감소되는 것이 관찰되었지만 임신 120일령태아에서는 그 수가 105일령태아보다 약간 증가한 양상을 나타내었다. 5. GFAP에 대한 끝뇌에서의 면역반응염색은 대부분의 연질막부위와 뇌실부위에서는 뚜렷했지만 그 사이에서는 임신 95일령태아에서 120일령태아까지 감소하는 양상이 관찰되었다. 이러한 현상들은 면역반응세포가 대뇌피질과 대뇌수질에서는 빠르게 발달하고 있었지만 뇌실부위는 늦게 발달하고 있음을 알 수 있었다.
GFAP (Glial Fibrillary Acidic Protein) was one of the intermediate filament group and used as an astrocyte marker. The numerous studies about GFAP immunoreactive cell’s distribution were investigated for fetus, neonate and aged brains. There are several reports about that GFAP immunoreactive cells were appeared at early fetus or after birth. In cases of mammalian fetus radial glia cells migrated toward pial surface at early stage and revealed GFAP immunoreactivity by the immunostain. But in cases of rodents, they migrated last gestation or after birth. This study, the GFAP immunoreactive cells’ localizations and distribution in the fetuses (the 30th, 45th, 60th, 90th, 95th, 105th 120th of gestation) and neonate telencephalon of Korean native goat were investigated by immunohistochemistry (ABC method). The results obtained in this study were summarized as followings; 1. Multipolar astrocytes of 60 days of gestation were found cerebral cortex, in 95 days of gestation were found cerebral medulla, in 105 days of gestation were found lateral ventricle. 2. Radial glial cell presented 45 days of gestation and process of GFAP immunoreactive was to stretch out from ventricular to pia mater. And the nonpolar immunoreactive cells were transformed bipolar immunoreactive cells and they were transformed to monopolar and multipolar immunoreactive cell. 3. The number of GFAP immunoreactive cells of a field were gradually increased from 45 days of gestation till 90 days of gestation and decreased from 90 days of gestation till 105 days of gestation. But in 120 days of gestation and newborn were slightly increased. 4. Immunoreactivity of GFAP immunoreactive cells were gradually decreased from 95 days of gestation till 120 days of gestatioin. However, most pia mater areas and ventricles had high immunoreactivity and medulla part had low immunoreactivity. These results were suggested that radial glial cell of cerebral cortex and cerebral medulla were developed faster than lateral ventricle.
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