질경이 추출물의 항산화 효과 및 각질세포 보호효과

Antioxidant effects of Plantago asiatica and protective effects on Human HaCaT Keratinocyte

This study was carried out to investigate antioxidative effects of Plantago asiatica and protective effects on Human HaCaT keratinocyte. The extracts were prepared by ethanol extraction method. The antioxidant activities of extracts was used the method of DPPH radical, total phenolics and flavonoids contents. In our results, treatment with Plantago asiatica did not show cytotoxicity on HaCaT keratinocyte beneath the concentration of 20, 50 ㎍/㎖. The DPPH radical scavenging activity of the aerial part of Plantago asiatica showed a considerable IC50 value (170.4 ㎍/㎖) and it was higher than that of BHT standard oxidant. The contents of total phenolics (174.0 ㎍/㎖) and flavonoids (130.9 ㎍/㎖). Plantago asiatica showde protective effect against cell death of HaCaT cell induced by hydrogen peroxide, rotenone, paraquat significantly. These results suggested that Plantago asiatica may have antioxidantic action in human skin and also suggest the possibility as cosmetic material.