본 연구에서는 신장 질병 발병의 주요한 세포인 신장 혈관사이 세포에 녹차 및 녹차 주요 성분인 EGCG, ECG, EGC 및 EC 처리 하였을 때 H2O2에 의해서 야기되는 세포의 세포사멸 증가, 산화성 스트레스증가 및 GSH 함량 감소가 차단되는 것을 확인하였다. 아울러 신장 질병 발병의 주요 지표인 TGF-1의 경우도 H2O2에 의해서 증가하였으며 이들 역시 녹차 및 녹차 주요 성분인 EGCG, ECG, EGC 및 EC 처리 하였을 때 차단되었다. 결론적으로 녹차의 catechin성분은 H2O2에 의한 신장 혈관사이 세포의 산화성 스트레스 증가 작용, GSH 함량 감소 및 TGF-1 발현 증가 작용 등을 차단하여 세포사멸을 예방하는 것으로 나타났다.
Green tea (GT) and its catechin components have been reported to have beneficial effect in human health. Major components of green tea are epigallocatechin-3-gallate (EGCG), epigallocatechin (EGC), epicatechin gallate(ECG), and epicatechin (EC). Onset of renal glomerulosclerosis is associated with the dysfunction of mesangial cells, such as the death of mesangial cells. Oxidative stress plays an pivotal role in the development of diabetic nephropathy. Thus, this study examined the effect of green tea extracts (GTE) and its major components (EGCG, EGC, ECG, and EC) against hydrogen peroxide-induced dysfunction of cultured rat mesangial cells. In the present study, treatment of hydrogen peroxide induced the increase of cell death and lipid peroxide (LPO) formation in a dose dependent manner in rat mesangial cells. The treatment of H2O2 also decreased GSH contents and increased TGF-β1 secretion. The treatment of GTE (10 mg/mL) prevented hydrogen peroxide-induced cell death, LPO formation, GSH contents and TGF-β1 secretion in mesangial cells. In addition, the treatment of EGCG, EGC, ECG, EC also prevented the hydrogen peroxide-induced cell death, LPO formation, GSH contents and TGF-β1 secretion. Among them, EGCG is a most powerful agent against hydrogen peroxide. In conclusion, GT blocked hydrogen peroxide-induced dysfunction of rat mesangial cells.
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