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대장균 박테리오페리틴의 항산화활성

The Antioxidant Activity of Bacterioferritin from Escherichia coli

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Bacterioferritin (BFR), an iron storage protein, takes up iron in the ferrous form and stores it within its central cavity as a hydrated ferric oxide mineral. The antioxidant activity of BFR against the metal catalyzed oxidation systems comprised of ferric ion and reducing agents was investigated. BFR of Escherichia coli was overexpressed and purified by ion-exchange and gel chromatographies. The protein inhibited the inactivation of glutamine synthetase caused by metal catalyzed oxidation system comprised of either dithiothreitol or ascorbate as a reducing agent.

Abstract

Ⅰ. 서론

Ⅱ. 재료 및 방법

Ⅲ. 결과

Ⅳ. 고찰

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