대장균 페리틴의 항산화 활성

The Antioxidant Activity of Ferritin of Escherichia coli

Ferritin (Ftn), an iron storage protein, takes up iron in the ferrous form and stores it within its central cavity as a hydrated ferric oxide mineral. The antioxidant activity of Ftn against the metal catalyzed oxidation systems comprised of ferric ion or cupric ion and reducing agents was investigated Ftn of Escherichia coli was overexpressed and purified by ion-exchange and gel chromatographies. The protein inhibited the inactivation of glutamine synthetase caused by metal catalyzed oxidation system comprised of either dithiothreitol or ascorbate as a reducing agent. However, removal activity of H₂O₂ by Ftn wasn’t detected.