다래 (Actinidia arguta) 유래 원형질체로부터 식물체 재분화

Protoplasts isolated from the embryogenic cell suspension cultures of Actinidia arguta were cultured to the green cell clusters. After 6-8 weeks, when microcalli formed to a diameter of about 2 ㎜, they were respectively transferred to solid MSB medium. Plant regeneration from calluses was carried out on MSB medium with 0.6 mM myo-inositol, 0.27% (w/v) Phytagel, 3% (w/v) glucose, and 0.5% activated charcoal. Well rooted plants were transferred to the soil. Forty two plant lines were selected and regenerated among the green cell clusters. Protoplast-derived plant lines had an similar floral and leaf morphology when compared to the parents.