질경이 seed 추출물의 항산화 활성 및 미백작용에 관한 연구

A study on Anti-oxidant activity and whitening action of Plantago asiatica L. seed extract

This study has attempted to find out if Plantago asiatica L. seed extract can be used as an ingredient in anti-aging and whitening cosmetics. According to a test on cytotoxicity using B16 melanoma cell, 100.9%, 87.4%, and 81.8% of cell survival rates were observed. Therefore, it has been confirmed that there is no cytotoxicity within the range of concentrations applied. In addition, it appears that it is a safe and effective skin-care material. According to measurement on DPPH radical scavenging activity with a goal of examining the antioxidant activity of the extract, no particular scavenging activity was found at 1㎍/㎖ or 101㎍/㎖. At 100㎍/㎖, on the contrary, 13.1% of antioxidant activity was detected. The ROS of hydrogen peroxide, which is created in the cell, has been measured using DCF-DA fluorescent material. According to observation of the inhibiting effect of ROS, which is created by silica, the creation of ROS has been strongly inhibited from the concentration-dependent aspect. At the highest concentration (100㎍/㎖), 53.8% of antioxidant activity was observed. According to measurement of L-dopa with a goal of figuring out the effect of Plantago asiatica L. seed extract on tyrosinase activity, no particular result was found. According to measurement of tyrosinase activity using B16 melanoma cell, on the contrary, minor inhibition of the activity was detected from a concentration-dependent perspective. At the highest concentration (100㎍/㎖), 15.8% of enzyme activation inhibition rate was observed. Based on the results above, it is likely that Plantago asiatica L. seed extract has a potential to be a natural antioxidant and whitening ingredient in the cosmetic industry. In addition, it could be available for medicinal purposes.