녹차의 고포도당에 의한 간세포 사멸 및 산화성 스트레스 예방효과

Green Tea Catechins Protect High Glucose-Induced Cell Death and Oxidative Stress in Human Hepatocyte Cell Line

본 연구에서는 간세포주인 HepG2 세포를 이용하여 녹차 및 녹차 주요 성분인 EGCG 및 EGC 처리 하였을 때 고포도당에 의해서 야기되는 간세포 세포사멸 증가, 산화성 스트레스증가 및 GSH 함양 감소작용이 차단되는 것을 확인하였다. 결론적으로 녹차의 EGCG를 주성분으로 하는 catechin 성분이 고포도도당에 의한 간 세포의 산화성 스트레스 증가 작용 및 GSH 함양 감소 작용등을 차단하여 간 세포사멸을 예방하는 것으로 나타났다.

Green tea is generally consumed in the World, since the administration of green tea (GT) is implicated in the decrease of onset of diverse diseases in human. Major components are epigallocatechin-3-gallate (EGCG), epigallocatechin(EGC), epicatechin gallate (ECG), and epicatechin (EC). Dysfunction of hepatocyte are associated with the development of diverse diseases such as diabetes. Hyperglycemia has been suggested to be a primary factor in the development of diabetes mellitus. Thus, this study examined the effect of green tea extract and its major components (EGCG, EGC) against 25 mM glucose-induced dysfunction of cultured HepG2 cells, Human hepatocyte cell line. In the present study, treatment of 25 mM glucose induced the increase of cell death and lipid peroxide formation in a dose dependent manner. The treatment of green tea extracts (GTE, 10 mg/mL) prevented 25 mM glucose-induced cell death and lipid peroxide formation in HepG2 cells. cells. In addition, the treatment of EGCG and EGC also prevented 25 mM glucose-induced cell death and LPO formation. Moreover, GTE, EGCG, and EGC prevented 25 mM glucose-induced GSH contents. Among them, EGCG is most powerful agents against 25 mM glucose. In conclusion, GT blocked 25 mM glucose-induced dysfunction of hepatocytes.