블루베리 잎 추출물의 항산화 및 항염증 효과

Anti-oxidant and Anti-inflammatory Effects of Extracts from Blueberry(Vaccinium ashei) Leaf

This study investigated the anti-oxidative and inhibitory activity of tyrosinase and the anti-inflammatory effects of blueberry leaf extracts for use as a functional ingredient in cosmetic products. Extraction yields via hot water and ethanol were 19.20% and 17.37%, respectively. As a results of measuring anti-oxidation, the electron donating activities of both hot water- and ethanol-extracts increased in a concentration-dependent manner, and showed 65.15% and 91.87% at 500 ㎍/㎖, respectively. The SOD-like activities were higher at the hot water extract. In the measurement of ABTS radical cation decolorization, both water- and ethanol-extracts showed a similar effect. Regarding the inhibitory activity of tyrosinase, the ethanol extract was significantly higher(83.43%) than the hot water extract(28.45%) at 1,000 ㎍/㎖. In these results, the topical application of blueberry leaf extracts to the epidermis ameliorated TPA-induced irritant contact dermatitis. Substantial reductions were observed in skin thickness and tissue weight, neutrophil-mediated myeloperxidase activity, and various histopathological indicators. These results suggest that an ethanol extract of blueberry leaf may act as a natural ingredient for functional cosmetics.