마디풀(Polygonum aviculare L.) 추출물의 대식구 면역증강 효과

Immune-stimulating Effects of Polygonum aviculare L. Extract on Macrophages

In this study we demonstrated whether the extract of Polygonum aviculare L. (PAE) can be applied to the immune-stimulating responses in macrophages (Raw 264.7 cells). Cell viability was determined by WST-8 assay, and all four doses of PAE (5, 10, 20, and 40 µg/ml) had no significant cytotoxicity during the entire experimental period. PAE ncreased the production of inducible nitric oxide synthase (iNOS) and nitric oxide (NO), and mRNA expressions and protein levels of pro-inflammatory cytokines(tumor necrotic factor (TNF)-α, interleukin (IL)-1β and IL-6) in the same cells. These immune-stimulating activities of PAE were found to be caused by the stimulation of NFκB signal and phosphorylation of MAP kinases (p38, ERK and JNK).