위령선 추출물의 항염활성 및 멜라닌 생성 억제효과

Anti-inflammatory Effect and Inhibition of Melanin Biosynthesis of Clematis mandshurica

Clematis mandshurica (Ranunculaceae) has traditionally been used as a remedy for antidiuretic, antifungal, rheumatic conditions and alleviate pain. We carried out to evaluate the anti-oxidative effect, anti-inflammatory effect and anti-melanogenic effect of ethanol extract and solvent fractions of Clematis mandshurica. The ethanol extract and the dichlo-romethane fraction of Clematis mandshurica showed an anti-oxidative effect in DPPH assay, the inhibitory activity of nitric oxide (NO) production in lipopolysaccharide (LPS) activated RAW 264.7 cell, and melanin synthesis and tyrosinase activity of B16F10 melanoma cells. They reduced NO production and melanin content in a dose-dependent manner at con-centrations of 2.5~10 µg/ml. They also suppressed iNOS and tyrosinase protein and m-RNA expressions dose dependently,assayed by western blot analysis and RT-PCR experiment.