LC-MS/MS를 이용한 당귀수산 추출물 중 17종 성분의 함량분석

Quantitative Analysis of the Seventeen Marker Components in Dangguisu-san Using Ultra-performance Liquid Chromatography Coupled to Electrospray Ionization Tandem Mass Spectrometry

Dangguisu-san is a well-known traditional Korean herbal medicine prescription and has been widely used to treat ecchymosis, blood stagnation, and pain resulting from physical shock in Korea. In this study, an ultra-performance liq-uid chromatography-electrospray ionization-mass spectrometer method was established for the simultaneous determination of the 17 biomarker components in Dangguisu-san. All analytes were separated on an UPLC BEH C18 (100×2.1 mm,1.7 μm) column and maintained at 45 oC. The mobile phase consisted of two solvent systems, 0.1% (v/v) formic acid in water(A) and acetonitrile (B) by gradient flow. The injection volume was 2.0 μl and the flow rate was 0.3 ml/min with detection at mass spectrometer. Calibration curves of the 17 biomarker components were acquired with r 2 values ≥0.9951. The values of limit of detection and quantification of all analytes were 0.02~6.32 ng/ml and 0.05~18.95 ng/ml, respectively. The amounts of the 17 components in Dangguisu-san sample were 3.17~13,224.50 μg/g.