크로마토그래프법에 의한 Hippuran131-I의 순도시험

Purity Test of Hippuran-131I by Chromatography

The purified o-iodohippuric acid-131I, o-iodobenzoic acid-131I, sodium iodide-131I and mixtures of those compounds were applied to radio paper or radio thin layer chromatography to select the developing solvents with useful separation efficiency. The separation efficiencies were checked by radiochromatogram scannings. It has been found that the two dimensional radio paper chromatography using the solvent systems of 8% phenol and butanol-glacial acetic acid-water (4 : 1 : 1 v/v) and the radio thin layer chromatography using alumina gel plate and butanol-glacial acetic acid-water (4 : 1 : I v/v) are both efficient. In this method, the Rf value of o-iodohippuric acid-131I, o-iodobenzoic acid-131I and the unbound 131I is 0.48, 0.85 and 0.15, respectively.