인삼 사포닌이 백서 장점막 Na+, K+-ATPase에 미치는 영향에 관한 연구

A Study on the Effect of Ginseng Saponin on Rat Intestinal Mucosal Na+, K+-ATPase

We have studied the effect of ouabain, total ginseng saponin, panax saponin C (protopanaxatriol derivative) and ginsenoside Rb1 (protopanaxadiol derivative) on Na+, K+-ATPase and Mg++-ATPase activity in rat intestinal microsomal fraction. Intestinal microsomal fraction was prepared by the method of Robinson and ATPase activities were determined by the method of King. The Na+, K+-ATPase activities were inhibited by 90.1% and 51.1% respectively at the concentration of 10-3M and 10-4M ouabain. The results are consistent with those of Robinson. The Na+, K+-ATPase activities were increased by 14.3 % and 10.0% respectively at the concentration of 10-4g/ml and 10-5g/ml total ginseng saponin. Panax saponin C obtained by the method of Han and ginsenoside Rb, obtained by the method of Shibata were used. The Na+, K+-ATPase activities were increased in the presence of panax saponin C and the increased activity with panax saponin C was greater than that with total ginseng saponin. On the other hand ginsenoside Rb1 showed an inhibitory effect on Na+, K+-ATPase. Total ginseng saponin, panax saponin C and ginsenoside Rb, had no effect on Mg++-ATPase. Therefore, it may be concluded that total ginseng saponin has dual effects on microsomal Na+, K+-ATPase, that is, panax saponin C exhibits stimulatory action, whereas ginsenoside Rb1 shows inhibitory action.