사람 글로빈의 Chain Anomaly 결정에 관한 연구

Studies on the Chain Anomaly Determination of Human Globins

We studied on the separation of alpha- and beta-chain globins, the conditions of paper electrophoresis and mobility of globins in paper electrophoresis to determine their chain anomaly. Acidic-acetone mothod of Wilson and Smith was found to be more effecient for separating alpha-and beta-chain globins than trichloroacetic acid method of Hayashi. The conditions of paper electrophoresis were current of 20v/cm and 0.6mA/cm for three hours at room temperature. In paper electroporesis, the mobility of a-chain globin towards the cathode was greater than that of @-chain globin. The results showed that chain anomaly of alpha- and beta-chain globins can be determined by the method of paper electrophoresis.