가토에 있어서 Capsaicin의 흡수 및 배설에 관한 연구

Study on the Absorption and Excretion of Capsaicin in Rabbits

Quantitative determination of capsaicin in biological fluid was investigated. The pharmacokinetic study of capsaicin in rabbits was performed by high-pressure liquid chromatography, equipped with a microparticulate reversed-phase column and a fixed wavelength detector. Elution was carried out using methanol-water(70:30). It allows the quantitative determination at 8~400ng level. When single dose of capsaicin(4mg/kg) was given to rabbits intravenously, the elimination phase was extremely short with average half-life to 17.35 minute. Urine excretion of capsaicin itself during first 2 hours after intravenous administration (4mg/kg) was 0.004~0.04% of the administered amount. The maximum plasma concentration of capsaicin after oral administration (300mg/kg) was 4 X 1O-7g/ml at 40 minutes. The LD50 of capsaicin in mouse was 0.40mg/kg (i.v.) and 47.2mg/kg (p.o.) which was determined by Litchfield and Wilcoxon''s method, suggesting that the gastrointestinal absorption of capsaicin is poor.