학술저널
혈청 3,5,3''-Triiodothyronine 측정을 위한 효소-면역 분석의 개발 연구
Development of Homogeneous Enzyme Immunoassay for Serum 3,5,3''-Triiodothyronine Determination
- 대한약학회
- 약학회지
- 제27권 제2호 (1983년)
-
1983.06117 - 124 (8 pages)
- 0
For development of EMIT-T3 assay, the conjugation of 3, 5, 3''-triiodothyroformic acid NHS ester to G6PDH was attempted in various reaction conditions. Up to now, the best conjugation condition was the ratio of T3-NHS:G6PDH=100 in 25% carbitol-Tris buffer at pH 9, OoC during overnight. The obtained T3-G6PDH conjugates usually had 20% residual enzyme activity which was inhibited by 40~70% with various anti-T3 antibodies. Utilizing the conjugate I and an antibody (S2633G), a useful standard curve for T3 assay was obtained in the range of 0 to 5ng/ml with 499 EMIT units of separation.
(0)
(0)