GC/FPD를 이용한 beta-락탐계 항생물질의 분석(II)

Determination of beta-Lactam Antibiotics by Gas-Chromatography with Flame Photometric Detector (II)

For gas chromatographic determination with the sulfur-specific flame photometric detector, nine beta-lactam antibiotics without alpha-amino group were esterified with methyl iodide and eight beta-lactam antibiotics with alpha-amino group were esterified with borontrifluoride-methanol complex and then N-benzoylated with benzoyl chloride. The gas chromatographic separation of these products was successfully carried out on various silicon polymers (OV-1, OV-101, OV-17, OV-225, and QF-1) coated on the acid washed, silanized diatomite. The structure of the esterified and N-benzoylated product was confirmed by mass spectrometer.